PfCRT GENE POLYMORPHISMS IN PLASMODIUM FALCIPARUM ISOLATES FROM MALARIA PATIENTS IN MALARIA ENDEMIC AREAS USING ACT AS STANDARD THERAPY

The pfcrt gene is a biomarker to determine the resistance of Plasmodium falciparum chloroquine and amodiaquine. When was switched ACT, it very likely that there will be an increase in wild type strains (pfcrt K76) due absence exposure chloroquine. Currently has not used for malaria treatment. aims this study were identify polymorphisms phylogenetic analysis isolates from patients Pesawaran Regency, Lampung Province. This research laboratory by analyzing blood samples which are stored biological materials (BBT). DNA isolation carried out using kit QIAmp Mini Qiagen, followed amplification appropriate primer. results sequencing then analyzed Mega 10. showed all had mutations codons 76 (K76T) 72 (C72S) gene. closely related other isolates. conclusion found PfCRT gene, although long been abandoned as antimalarial. sequenced pesawaran also